HxlB

• Description: 6-phospho-3-hexuloisomerase
  
  

Gene name	hxlB
Synonyms	yckF
Essential	no
Product	6-phospho-3-hexuloisomerase
Function	ribulose monophosphate pathway for formaldehyde fixation
Gene expression levels in SubtiExpress: hxlB
MW, pI	19 kDa, 5.791
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	tlpC, hxlA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HxlR regulon

The gene

Basic information

• Locus tag: BSU03450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: PHI subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VIV

• UniProt: P42404

• KEGG entry: [3]

• E.C. number: 5.3.1.27

Expression and regulation

• Operon: hxlA-hxlB PubMed

• Expression browser: hxlB PubMed

• Sigma factor:

• Regulation: induction by formaldehyde (HxlR) PubMed

• Regulatory mechanism: transcription activation by HxlR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Nobuo Kato, Hiroya Yurimoto, Rudolf K Thauer
The physiological role of the ribulose monophosphate pathway in bacteria and archaea.
Biosci Biotechnol Biochem: 2006, 70(1);10-21
[PubMed:16428816] [WorldCat.org] [DOI] (P p)

E J Taylor, S J Charnock, J Colby, G J Davies, G W Black
Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 8);1138-40
[PubMed:11468398] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)