LeuC
Revision as of 10:55, 14 May 2013 by 134.76.70.252 (talk)
- Description: 3-isopropylmalate dehydratase (large subunit)
Gene name | leuC |
Synonyms | |
Essential | no |
Product | 3-isopropylmalate dehydratase (large subunit) |
Function | biosynthesis of leucine |
Gene expression levels in SubtiExpress: leuC | |
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
MW, pI | 52 kDa, 6.127 |
Gene length, protein length | 1416 bp, 472 aa |
Immediate neighbours | leuD, leuB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, phosphoproteins
This gene is a member of the following regulons
CcpA regulon, CodY regulon, FsrA regulon, T-box, TnrA regulon
The gene
Basic information
- Locus tag: BSU28260
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O (according to Swiss-Prot)
- Protein family: LeuC type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- phosphorylated on Arg-81 PubMed
- Cofactor(s): contains an iron-sulfur cluster
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P80858
- KEGG entry: [3]
- E.C. number: 4.2.1.33
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed
- repressed by casamino acids PubMed
- expression is stimulated in the presence of glucose PubMed
- less expressed under conditions of extreme iron limitation (FsrA) PubMed
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References