ArtP

• Description: high affinity arginine ABC transporter (ATP-binding protein)
  
  

Gene name	artP
Synonyms	yqiX
Essential	no
Product	high affinity arginine ABC transporter (ATP-binding protein)
Function	arginine uptake
Gene expression levels in SubtiExpress: artP
Interactions involving this protein in SubtInteract: ArtP
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	28 kDa, 5.086
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	artQ, yqiW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

The gene

Basic information

• Locus tag: BSU23980

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(D) for Arg: 43 nM PubMed
  • K(D) for Lys: 657 myM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ArtR-ArtQ-ArtP PubMed
  • McpC-ArtP, for chemotaxis to lysine and arginine PubMed

• Localization:
  • attached to the membrane via ArtQ PubMed
  • extracellular (signal peptide) PubMed, membrane PubMed

Database entries

• Structure: 2Q2C (Geobacillus stearothermophilus)

• UniProt: P54535

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: artP-artQ-artR PubMed

• Expression browser: artP PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

George D Glekas, Brendan J Mulhern, Abigail Kroc, Keegan A Duelfer, Victor Lei, Christopher V Rao, George W Ordal
The Bacillus subtilis chemoreceptor McpC senses multiple ligands using two discrete mechanisms.
J Biol Chem: 2012, 287(47);39412-8
[PubMed:23038252] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Sekowska, S Robin, J J Daudin, A Hénaut, A Danchin
Extracting biological information from DNA arrays: an unexpected link between arginine and methionine metabolism in Bacillus subtilis.
Genome Biol: 2001, 2(6);RESEARCH0019
[PubMed:11423008] [WorldCat.org] [DOI] (I p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)