CitT

• Description: two-component response regulator, regulation of citrate uptake
  
  

Gene name	citT
Synonyms	yflQ
Essential	no
Product	two-component response regulator
Function	regulation of citrate uptake
Gene expression levels in SubtiExpress: citT
Interactions involving this protein in SubtInteract: CitT
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	25 kDa, 6.362
Gene length, protein length	678 bp, 226 aa
Immediate neighbours	citS, yflP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

The CitT regulon:

• citM-yflN

The gene

Basic information

• Locus tag: BSU07590

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the promoter of the citM-yflN operon and activation of transcription in the presence of citrate PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by CitS on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • CitS-CitT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: citS-citT-yflP PubMed

• Expression browser: citT PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)