PbuG

• Description: hypoxanthin/ guanine permease
  
  

Gene name	pbuG
Synonyms	yebB
Essential	no
Product	hypoxanthin/ guanine permease
Function	hypoxanthine and guanine uptake
Gene expression levels in SubtiExpress: pbuG
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation)
MW, pI	46 kDa, 8.808
Gene length, protein length	1320 bp, 440 aa
Immediate neighbours	guaA, yebC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

• Locus tag: BSU06370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): PbuO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure: 2EEW (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)

• UniProt: O34987

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbuG PubMed

• Expression browser: pbuG PubMed

• Sigma factor:

• Regulation:
  • repressed by hypoxanthine and guanine, PurR-independent, (G-box)PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: