YcbF

• Description: glucarate dehydratase
  
  

Gene name	ycbF
Synonyms	gudD
Essential	no
Product	glucarate dehydratase
Function	glucarate utilization
Gene expression levels in SubtiExpress: ycbF
MW, pI	50 kDa, 5.594
Gene length, protein length	1365 bp, 455 aa
Immediate neighbours	ycbE, ycbG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

• Locus tag: BSU02490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: d-glucarate = 5-dehydro-4-deoxy-D-glucarate + H₂O (according to Swiss-Prot)

• Protein family: GlucD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42238

• KEGG entry: [3]

• E.C. number: 4.2.1.40

Expression and regulation

• Operon: ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ PubMed

• Expression browser: ycbF PubMed

• Sigma factor:

• Regulation: induced by D-glucarate/galactarate (YcbG) PubMed

• Regulatory mechanism: YcbG: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)