RnpA

• Description: protein component of RNase P, metal-dependent 5' end maturation of precursor tRNAs
  
  

Gene name	rnpA
Synonyms
Essential	yes PubMed
Product	protein component of RNase P (substrate specificity)
Function	cleavage of precursor sequences from the 5' ends of pre-tRNAs
Gene expression levels in SubtiExpress: rnpA
Interactions involving this protein in SubtInteract: RnpA
MW, pI	13 kDa, 10.804
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	spoIIIJ, rpmH
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, essential genes

The gene

Basic information

• Locus tag: BSU41050

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to Swiss-Prot)

• Protein family: rnpA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • RNR motif: residues 60-68, stabilizes complexes with both the RNA part of RNase P (rnpB) and with pre-tRNA (rnpB-RnpA-pre-tRNA) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • RnpA-RpmH
  • RnpA-RnpB PubMed
  • RnpA-CshA PubMed

• Localization:

Database entries

• Structure:
  • 1AF6 PubMed
  • 3OKB (complex of RnpA with the RNA RnpB and tRNA, from Thermotoga maritima) PubMed

• UniProt: P25814

• KEGG entry: [2]

• E.C. number: 3.1.26.5

Expression and regulation

• Operon:

• Expression browser: rnpA PubMed

• Sigma factor:

• Regulation:
  • expression transiently increases in the forespore PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Carol Fierke, University of Michigan, Ann Arbor, USA homepage
• Roland Hartmann, Marburg University, Germany homepage

References

Reviews

Roland K Hartmann, Markus Gössringer, Bettina Späth, Susan Fischer, Anita Marchfelder
The making of tRNAs and more - RNase P and tRNase Z.
Prog Mol Biol Transl Sci: 2009, 85;319-68
[PubMed:19215776] [WorldCat.org] [DOI] (P p)

J Kristin Smith, John Hsieh, Carol A Fierke
Importance of RNA-protein interactions in bacterial ribonuclease P structure and catalysis.
Biopolymers: 2007, 87(5-6);329-38
[PubMed:17868095] [WorldCat.org] [DOI] (P p)

Alexei V Kazantsev, Norman R Pace
Bacterial RNase P: a new view of an ancient enzyme.
Nat Rev Microbiol: 2006, 4(10);729-40
[PubMed:16980936] [WorldCat.org] [DOI] (I p)

Donald Evans, Steven M Marquez, Norman R Pace
RNase P: interface of the RNA and protein worlds.
Trends Biochem Sci: 2006, 31(6);333-41
[PubMed:16679018] [WorldCat.org] [DOI] (P p)

Alfredo Torres-Larios, Kerren K Swinger, Tao Pan, Alfonso Mondragón
Structure of ribonuclease P--a universal ribozyme.
Curr Opin Struct Biol: 2006, 16(3);327-35
[PubMed:16650980] [WorldCat.org] [DOI] (P p)

John Hsieh, Andy J Andrews, Carol A Fierke
Roles of protein subunits in RNA-protein complexes: lessons from ribonuclease P.
Biopolymers: 2004, 73(1);79-89
[PubMed:14691942] [WorldCat.org] [DOI] (P p)

Enno Hartmann, Roland K Hartmann
The enigma of ribonuclease P evolution.
Trends Genet: 2003, 19(10);561-9
[PubMed:14550630] [WorldCat.org] [DOI] (P p)

L A Kirsebom, A Vioque
RNase P from bacteria. Substrate recognition and function of the protein subunit.
Mol Biol Rep: 1995, 22(2-3);99-109
[PubMed:8901495] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed