Lgt

• Description: prolipoprotein diacylglyceryl transferase
  
  

Gene name	lgt
Synonyms	gerF, yvoC
Essential	no
Product	prolipoprotein diacylglyceryl transferase
Function	lipomodification of lipoproteins
Gene expression levels in SubtiExpress: lgt
Interactions involving this protein in SubtInteract: Lgt
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Protein secretion
MW, pI	30 kDa, 8.96
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	yvoD, hprK
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, germination, membrane proteins

The gene

Basic information

• Locus tag: BSU34990

Phenotypes of a mutant

• reduced ability of spores to germinate with alanine due ti reduced activity of the GerAA-GerAB-GerAC germinant receptor PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lgt family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerAC-Lgt to fix GerAC to diacylglycerol in the membrane PubMed
  • GerD-Lgt to fix GerD to diacylglycerol in the membrane PubMed

• Localization:
  • membrane associated PubMed

Database entries

• Structure:

• UniProt: O34752

• KEGG entry: [2]

• E.C. number: 2.4.99.-

Expression and regulation

• Operon: hprK-lgt-yvoD-yvoE-yvoF PubMed

• Expression browser: lgt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP851 (deletion of lgt-yvoD-yvoE-yvoF, replaced by aphA3), available in the Stülke lab
  • 1G22 ( lgt::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

PubMed

Original publications

Kerry-Ann V Stewart, Xuan Yi, Sonali Ghosh, Peter Setlow
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.
J Bacteriol: 2012, 194(12);3156-64
[PubMed:22493018] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Takao Igarashi, Barbara Setlow, Madan Paidhungat, Peter Setlow
Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis.
J Bacteriol: 2004, 186(10);2984-91
[PubMed:15126458] [WorldCat.org] [DOI] (P p)

S Leskelä, E Wahlström, V P Kontinen, M Sarvas
Lipid modification of prelipoproteins is dispensable for growth but essential for efficient protein secretion in Bacillus subtilis: characterization of the Lgt gene.
Mol Microbiol: 1999, 31(4);1075-85
[PubMed:10096076] [WorldCat.org] [DOI] (P p)

J Bengtsson, H Tjalsma, C Rivolta, L Hederstedt
Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein.
J Bacteriol: 1999, 181(2);685-8
[PubMed:9882689] [WorldCat.org] [DOI] (P p)

A Galinier, M Kravanja, R Engelmann, W Hengstenberg, M C Kilhoffer, J Deutscher, J Haiech
New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression.
Proc Natl Acad Sci U S A: 1998, 95(4);1823-8
[PubMed:9465101] [WorldCat.org] [DOI] (P p)

Lgt in other organisms

PubMed