McpB

• Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine
  
  

Gene name	mcpB
Synonyms
Essential	no
Product	methyl-accepting chemotaxis protein
Function	control of chemotaxis
Gene expression levels in SubtiExpress: mcpB
Interactions involving this protein in SubtInteract: McpB
MW, pI	71 kDa, 5.13
Gene length, protein length	1986 bp, 662 aa
Immediate neighbours	tlpA, tgl
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU31260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): McpA

Extended information on the protein

• Kinetic information: K(D) for asparagine: 14 myM PubMed

• Domains:

• Modification: methylated at residues Gln371, Glu630, and Glu637 PubMed

• Cofactor(s):

• Effectors of protein activity: asparagine (binds to the part of McpB that is exposed to the exterior) PubMed

• Interactions:
  • CheA-McpB PubMed
  • McpB-CheC PubMed

• Localization:
  • cell membrane PubMed
  • localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) PubMed

Database entries

• Structure:

• UniProt: P39215

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mcpB PubMed

• Expression browser: mcpB PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, McpB is present with 6,200 +/- 1,900 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: