SspC

• Description: small acid-soluble spore protein (minor alpha/beta-type SASP)
  
  

Gene name	sspC
Synonyms
Essential	no
Product	small acid-soluble spore protein (minor alpha/beta-type SASP)
Function	protection of spore DNA
Gene expression levels in SubtiExpress: sspC
MW, pI	7 kDa, 9.102
Gene length, protein length	216 bp, 72 aa
Immediate neighbours	yotB, yosX
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, SP-beta prophage

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU19950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2Z3X (in complex with DNA)

• UniProt: P02958

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sspC PubMed

• Expression browser: sspC PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ki Seog Lee, Daniela Bumbaca, Jeffrey Kosman, Peter Setlow, Mark J Jedrzejas
Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species.
Proc Natl Acad Sci U S A: 2008, 105(8);2806-11
[PubMed:18287075] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Daphna Frenkiel-Krispin, Rinat Sack, Joseph Englander, Eyal Shimoni, Miriam Eisenstein, Esther Bullitt, Rachel Horowitz-Scherer, Christopher S Hayes, Peter Setlow, Abraham Minsky, Sharon Grayer Wolf
Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.
J Bacteriol: 2004, 186(11);3525-30
[PubMed:15150240] [WorldCat.org] [DOI] (P p)

Jeffrey Kosman, Peter Setlow
Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA.
J Bacteriol: 2003, 185(20);6095-103
[PubMed:14526021] [WorldCat.org] [DOI] (P p)

Anjum Sohail, Christopher S Hayes, Pradeep Divvela, Peter Setlow, Ashok S Bhagwat
Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination.
Biochemistry: 2002, 41(38);11325-30
[PubMed:12234173] [WorldCat.org] [DOI] (P p)

C S Hayes, P Setlow
An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores.
J Bacteriol: 2001, 183(8);2662-6
[PubMed:11274127] [WorldCat.org] [DOI] (P p)

C S Hayes, E Alarcon-Hernandez, P Setlow
N-terminal amino acid residues mediate protein-protein interactions between DNA-bound alpha /beta -type small, acid-soluble spore proteins from Bacillus species.
J Biol Chem: 2001, 276(3);2267-75
[PubMed:11044450] [WorldCat.org] [DOI] (P p)

C S Hayes, B Illades-Aguiar, L Casillas-Martinez, P Setlow
In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species.
J Bacteriol: 1998, 180(10);2694-700
[PubMed:9573155] [WorldCat.org] [DOI] (P p)

Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212] [WorldCat.org] [DOI] (P p)

W L Nicholson, D X Sun, B Setlow, P Setlow
Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters.
J Bacteriol: 1989, 171(5);2708-18
[PubMed:2468649] [WorldCat.org] [DOI] (P p)

M J Connors, P Setlow
Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence.
J Bacteriol: 1985, 161(1);333-9
[PubMed:2981806] [WorldCat.org] [DOI] (P p)