CwlC

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  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlC
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function mother cell lysis
Gene expression levels in SubtiExpress: cwlC
MW, pI 27 kDa, 5.939
Gene length, protein length 765 bp, 255 aa
Immediate neighbours ymaB, spoVK
Sequences Protein DNA Advanced_DNA
Genetic context
CwlC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlC expression.png
























Categories containing this gene/protein

cell wall/ other, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU17410

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1X60 (peptidoglycan binding domain)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Masaki Mishima, Toshio Shida, Kazuto Yabuki, Ken-Ichi Kato, Junichi Sekiguchi, Chojiro Kojima
Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR.
Biochemistry: 2005, 44(30);10153-63
[PubMed:16042392] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
J Biol Chem: 2001, 276(30);28140-6
[PubMed:11375403] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

A Kuroda, Y Asami, J Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
J Bacteriol: 1993, 175(19);6260-8
[PubMed:8407798] [WorldCat.org] [DOI] (P p)