PyrB

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  • Description: aspartate carbamoyltransferase

Gene name pyrB
Synonyms
Essential no
Product aspartate carbamoyltransferase
Function pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrB
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 34 kDa, 5.341
Gene length, protein length 912 bp, 304 aa
Immediate neighbours pyrP, pyrC
Sequences Protein DNA Advanced_DNA
Genetic context
PyrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PyrB expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

  • Locus tag: BSU15490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate (according to Swiss-Prot)
  • Protein family: ATCase/OTCase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-303 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References