ClpE

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  • Description: ATP-dependent Clp protease-like (class III stress gene)

Gene name clpE
Synonyms ykvH
Essential no
Product ATP-dependent Clp protease-like
Function protein degradation
Gene expression levels in SubtiExpress: clpE
Interactions involving this protein in SubtInteract: ClpE
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 77 kDa, 5.135
Gene length, protein length 2097 bp, 699 aa
Immediate neighbours motA, ykvI
Sequences Protein DNA Advanced_DNA
Genetic context
ClpE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ClpE expression.png















Categories containing this gene/protein

proteolysis, heat shock proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

  • Locus tag: BSU13700

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATPase/chaperone
  • Protein family: AAA+ -type ATPase (IPR013093) InterPro (PF07724) PFAM

Extended information on the protein

  • Kinetic information:
  • Domains: AAA-ATPase PFAM
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed
    • forms foci coincident with nucleoid edges, usually near cell poles PubMed

ClpE.jpg

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: clpE::spec available from the Hamoen] Lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion: C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, Newcastle University, UK homepage

Your additional remarks

References

Reviews

Additional reviews: PubMed

Dorte Frees, Kirsi Savijoki, Pekka Varmanen, Hanne Ingmer
Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, Gram-positive bacteria.
Mol Microbiol: 2007, 63(5);1285-95
[PubMed:17302811] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed