ArgJ

• Description: N-acetylglutamate synthase
  
  

Gene name	argJ
Synonyms	argA, argE
Essential	no
Product	N-acetylglutamate synthase
Function	biosynthesis of arginine
Gene expression levels in SubtiExpress: argJ
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	43 kDa, 4.703
Gene length, protein length	1218 bp, 406 aa
Immediate neighbours	argC, argB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU11200

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate (according to Swiss-Prot)

• Protein family: argJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P36843

• KEGG entry: [3]

• E.C. number: 2.3.1.35

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF PubMed

• Expression browser: argJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC) PubMed

• Regulatory mechanism:
  • AhrC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)