GlpD

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  • Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)

Gene name glpD
Synonyms
Essential no
Product glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function glycerol utilization
Gene expression levels in SubtiExpress: glpD
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 62 kDa, 8.002
Gene length, protein length 1665 bp, 555 aa
Immediate neighbours glpK, pgcA
Sequences Protein DNA Advanced_DNA
Genetic context
GlpD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlpD expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

  • Locus tag: BSU09300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)
  • Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): FAD
  • Effectors of protein activity:

Database entries

  • Structure: 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant: QB5347 (cat), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

E Glatz, A Farewell, B Rutberg
The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli.
FEMS Microbiol Lett: 1998, 162(1);93-6
[PubMed:9595668] [WorldCat.org] [DOI] (P p)

Elisabeth Glatz, Martin Persson, Blanka Rutberg
Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
Microbiology (Reading): 1998, 144 ( Pt 2);449-456
[PubMed:9493382] [WorldCat.org] [DOI] (P p)

E Glatz, R P Nilsson, L Rutberg, B Rutberg
A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability.
Mol Microbiol: 1996, 19(2);319-28
[PubMed:8825777] [WorldCat.org] [DOI] (P p)

C Holmberg, L Rutberg
An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription.
Mol Microbiol: 1992, 6(20);2931-8
[PubMed:1479885] [WorldCat.org] [DOI] (P p)