PhoB

• Description: alkaline phosphatase III
  
  

Gene name	phoB
Synonyms	phoAIII
Essential	no
Product	alkaline phosphatase III
Function	aquisition of phosphate upon phosphoate starvation
Gene expression levels in SubtiExpress: phoB
Metabolic function and regulation of this protein in SubtiPathways: Folate
MW, pI	50 kDa, 5.895
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	ydhF, fra
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, sporulation proteins

This gene is a member of the following regulons

PhoP regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU05740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family: alkaline phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): PhoA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P19405

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon: phoB-ydhF PubMed

• Expression browser: phoB PubMed

• Sigma factor: SigA, SigE PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A767 ( phoB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: