GlpT

• Description: glycerol-3-phosphate permease
  
  

Gene name	glpT
Synonyms	ybeE
Essential	no
Product	glycerol-3-phosphate permease
Function	glycerol-3-phosphate uptake
Gene expression levels in SubtiExpress: glpT
MW, pI	49 kDa, 9.366
Gene length, protein length	1332 bp, 444 aa
Immediate neighbours	glpQ, ybeF
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, utilization of lipids, membrane proteins

This gene is a member of the following regulons

CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU02140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC37A family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37948

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glpT-glpQ PubMed

• Expression browser: glpT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (6.2-fold) (CcpA) PubMed
  • induced by glycerol (GlpP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination via a protein-dependent RNA switch PubMed

• Additional information:

Biological materials

• Mutant: QB5437 (ermC), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

R P Nilsson, L Beijer, B Rutberg
The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);723-30
[PubMed:8012593] [WorldCat.org] [DOI] (P p)