FloA

• Description: flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime
  
  

Gene name	floA
Synonyms	yqfA
Essential	no
Product	flottilin-like protein
Function	resistance against sublancin
Gene expression levels in SubtiExpress: floA
Interactions involving this protein in SubtInteract: FloA
MW, pI	35 kDa, 4.917
Gene length, protein length	993 bp, 331 aa
Immediate neighbours	yqfB, yqeZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane dynamics, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU25380

Phenotypes of a mutant

• • reduced protein secretion PubMed
  • a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
  • a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
  • a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0365 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FloA-FtsH PubMed
  • FloT-FloA PubMed

• Localization:
  • membrane PubMed
  • co-localizes with FloT in discrete foci in the membrane PubMed
  • forms focal structure in the cytoplasma membrane PubMed

Database entries

• Structure:

• UniProt: P54466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqeZ-floA-yqfB PubMed

• Expression browser: floA PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed under stress conditions (SigW) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: