YabT

• Description: Ser/Thr kinase, controls DNA integrity during spore development
  
  

Gene name	yabT
Synonyms
Essential	no
Product	Ser/Thr kinase
Function	control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI	37 kDa, 9.943
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yabS, tilS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU00660

Phenotypes of a mutant

• increased sensitivity to DNA damage during spore development PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylation of RecA on Ser-2 PubMed

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • autophosphorylation is stimulated by non-specific binding to DNA PubMed

• Interactions:
  • YabT-RecA PubMed

• Localization:
  • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

• Structure:

• UniProt: P37562

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yabT PubMed

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP577 (erm), available in Jörg Stülke's lab

• Expression vector:
  • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab

• lacZ fusion:
• translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody: