List of plasmids

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

pAC5: translational lacZ-fusions (chloramphenicol resistence)
pAC6: transcriptional lacZ-fusions (cat resistence)
pAC7: translational lacZ-fusions (kanamycin resistence)
pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci PubMed

pBP43: integrative plasmid for the fusion of monomeric GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, this is the preferred plasmid for constructing GFP fusions!
pGP1871: integrative plasmid for the fusion of YFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter
pGP1080: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, allows express of the downstream gene by the P(spac) promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!
pGP1870: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!

pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter

pBP26: expression of YFP in B. subtilis and in E. coli
pBP27: expression of CFP in B. subtilis and in E. coli
pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
pGP886: integration vector (integrates in xkdE); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
pGP888: integration vector (integrates in ganA); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
pGP1459: integration vector (integrates in ganA) analogous to pGP380; expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
pGP1460: integration vector (integrates in ganA) analogous to pGP382; expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
pGP1389: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter

pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein