PGP172

pGP172: click to enlarge

pGP172 cloning region: click to enlarge

• vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

• host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

• constructed in the Stülke lab

• in E. coli: ampicillin resistance

• based on pET3C

• similar plasmid: pGP574

• sequencing primer:
  • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
  • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

• important: EcoRI, although mentioned in the MCS, is not unique!