Rnc

• Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
  
  

Gene name	rnc
Synonyms	rncS
Essential	yes
Product	endoribonuclease III
Function	cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	28 kDa, 8.076
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	acpA, smc
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU15930

Phenotypes of a mutant

• essential PubMed
• the rnc gene can be deleted in strains cured of the Skin element and SPß prophage (or upon deletion of the toxin genes txpA and yonT) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
  • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
  • degradation of yonT mRNA-as-yonT RNA duplex (encoded on the SP-beta prophage) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P51833

• KEGG entry: [3]

• E.C. number: 3.1.26.3

Expression and regulation

• Operon: rnc-smc-ftsY PubMed

• Expression browser: rnc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

References

Original publications

Durand S, Gilet L, Condon C  
The essential function of B. subtilis RNase III is to silence foreign toxin genes. 
PLoS Genet. 2012 8(12): e1003181.
PubMed:23300471