Rnc

• Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
  
  

Gene name	rnc
Synonyms	rncS
Essential	yes
Product	endoribonuclease III
Function	cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	28 kDa, 8.076
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	acpA, smc
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU15930

Phenotypes of a mutant

• essential PubMed
• the rnc gene can be deleted in strains cured of the Skin element and prophage SPß (or upon deletion of the toxin genes txpA and yonT) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
  • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
  • degradation of yonT mRNA-as-YonT RNA duplex (encoded on the SP-beta prophage) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P51833

• KEGG entry: [3]

• E.C. number: 3.1.26.3

Expression and regulation

• Operon: rnc-smc-ftsY PubMed

• Expression browser: rnc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

Fabian M Commichau, Jörg Stülke
A mystery unraveled: essentiality of RNase III in Bacillus subtilis is caused by resident prophages.
PLoS Genet: 2012, 8(12);e1003199
[PubMed:23300472] [WorldCat.org] [DOI] (I p)

Ian J MacRae, Jennifer A Doudna
Ribonuclease revisited: structural insights into ribonuclease III family enzymes.
Curr Opin Struct Biol: 2007, 17(1);138-45
[PubMed:17194582] [WorldCat.org] [DOI] (P p)

Xinhua Ji
Structural basis for non-catalytic and catalytic activities of ribonuclease III.
Acta Crystallogr D Biol Crystallogr: 2006, 62(Pt 8);933-40
[PubMed:16855311] [WorldCat.org] [DOI] (P p)

Djamel Drider, Ciarán Condon
The continuing story of endoribonuclease III.
J Mol Microbiol Biotechnol: 2004, 8(4);195-200
[PubMed:16179796] [WorldCat.org] [DOI] (P p)

R A Srivastava, N Srivastava
The multifaceted roles of the RNA processing enzyme ribonuclease III.
Indian J Biochem Biophys: 1996, 33(4);253-60
[PubMed:8936814] [WorldCat.org] (P p)

Original publications

Sylvain Durand, Laetitia Gilet, Ciarán Condon
The essential function of B. subtilis RNase III is to silence foreign toxin genes.
PLoS Genet: 2012, 8(12);e1003181
[PubMed:23300471] [WorldCat.org] [DOI] (I p)

Sylvain Durand, Laetitia Gilet, Philippe Bessières, Pierre Nicolas, Ciarán Condon
Three essential ribonucleases-RNase Y, J1, and III-control the abundance of a majority of Bacillus subtilis mRNAs.
PLoS Genet: 2012, 8(3);e1002520
[PubMed:22412379] [WorldCat.org] [DOI] (I p)

Iñigo Lasa, Alejandro Toledo-Arana, Alexander Dobin, Maite Villanueva, Igor Ruiz de los Mozos, Marta Vergara-Irigaray, Víctor Segura, Delphine Fagegaltier, José R Penadés, Jaione Valle, Cristina Solano, Thomas R Gingeras
Genome-wide antisense transcription drives mRNA processing in bacteria.
Proc Natl Acad Sci U S A: 2011, 108(50);20172-7
[PubMed:22123973] [WorldCat.org] [DOI] (I p)

Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666] [WorldCat.org] [DOI] (I p)

M A Herskovitz, D H Bechhofer
Endoribonuclease RNase III is essential in Bacillus subtilis.
Mol Microbiol: 2000, 38(5);1027-33
[PubMed:11123676] [WorldCat.org] [DOI] (P p)

Hiroshi Kakeshita, Akihiro Oguro, Reiko Amikura, Kouji Nakamura, Kunio Yamane
Expression of the ftsY gene, encoding a homologue of the alpha subunit of mammalian signal recognition particle receptor, is controlled by different promoters in vegetative and sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 10);2595-2603
[PubMed:11021934] [WorldCat.org] [DOI] (P p)

A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377] [WorldCat.org] [DOI] (P p)

W Wang, D H Bechhofer
Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci.
J Bacteriol: 1997, 179(23);7379-85
[PubMed:9393702] [WorldCat.org] [DOI] (P p)

S Mitra, D H Bechhofer
Substrate specificity of an RNase III-like activity from Bacillus subtilis.
J Biol Chem: 1994, 269(50);31450-6
[PubMed:7527387] [WorldCat.org] (P p)

A T Panganiban, H R Whiteley
Bacillus subtilis RNAase III cleavage sites in phage SP82 early mRNA.
Cell: 1983, 33(3);907-13
[PubMed:6409421] [WorldCat.org] [DOI] (P p)