NupG

• Description: purine nucleoside transporter
  
  

Gene name	nupG
Synonyms	yxjA
Essential	no
Product	purine nucleoside transporter
Function	purine uptake
Gene expression levels in SubtiExpress: nupG
MW, pI	43 kDa, 7.757
Gene length, protein length	1191 bp, 397 aa
Immediate neighbours	yxjB, yxiT/1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box

The gene

Basic information

• Locus tag: BSU39020

Phenotypes of a mutant

• a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC28A transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: P42312

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nupG

• Expression browser: nupG PubMed

• Sigma factor:

• Regulation:
  • expression activated by glucose (4.3 fold) PubMed
  • repressed by hypoxanthine and guanine, PurR-independent, G-boxPubMed

• Regulatory mechanism: G-box: transcriptional termination/ antitermination riboswitch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Abraham L Sonenshein
CodY-mediated regulation of guanosine uptake in Bacillus subtilis.
J Bacteriol: 2011, 193(22);6276-87
[PubMed:21926227] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)