SecA
- Description: preprotein translocase subunit (ATPase)
| Gene name | secA |
| Synonyms | div, div-341, ts-341 |
| Essential | yes PubMed |
| Product | preprotein translocase subunit (ATPase) |
| Function | protein secretion |
| Gene expression levels in SubtiExpress: secA | |
| Interactions involving this protein in SubtInteract: SecA | |
| Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
| MW, pI | 95 kDa, 5.34 |
| Gene length, protein length | 2523 bp, 841 aa |
| Immediate neighbours | prfB, yvyD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein secretion, essential genes, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35300
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP -> ADP + Pi + preprotein translocation
- Protein family: SecA family (according to Swiss-Prot)
- Paralogous protein(s): none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range
Extended information on the protein
- Kinetic information:
- Domains: nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
- Modification:
- Cofactor(s): magnesium
- Effectors of protein activity: anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) PubMed
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- UniProt: P28366
- KEGG entry: [3]
- E.C. number:
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Benedikt W Bauer, Tom A Rapoport
Mapping polypeptide interactions of the SecA ATPase during translocation.
Proc Natl Acad Sci U S A: 2009, 106(49);20800-5
[PubMed:19933328]
[WorldCat.org]
[DOI]
(I p)
Giorgos Gouridis, Spyridoula Karamanou, Ioannis Gelis, Charalampos G Kalodimos, Anastassios Economou
Signal peptides are allosteric activators of the protein translocase.
Nature: 2009, 462(7271);363-7
[PubMed:19924216]
[WorldCat.org]
[DOI]
(I p)
Jochen Zimmer, Tom A Rapoport
Conformational flexibility and peptide interaction of the translocation ATPase SecA.
J Mol Biol: 2009, 394(4);606-12
[PubMed:19850053]
[WorldCat.org]
[DOI]
(I p)
Jochen Zimmer, Yunsun Nam, Tom A Rapoport
Structure of a complex of the ATPase SecA and the protein-translocation channel.
Nature: 2008, 455(7215);936-43
[PubMed:18923516]
[WorldCat.org]
[DOI]
(I p)
Haiyuan Ding, John F Hunt, Ishita Mukerji, Donald Oliver
Bacillus subtilis SecA ATPase exists as an antiparallel dimer in solution.
Biochemistry: 2003, 42(29);8729-38
[PubMed:12873133]
[WorldCat.org]
[DOI]
(P p)
John F Hunt, Sevil Weinkauf, Lisa Henry, John J Fak, Paul McNicholas, Donald B Oliver, Johann Deisenhofer
Nucleotide control of interdomain interactions in the conformational reaction cycle of SecA.
Science: 2002, 297(5589);2018-26
[PubMed:12242434]
[WorldCat.org]
[DOI]
(I p)
J P Müller, J Ozegowski, S Vettermann, J Swaving, K H Van Wely, A J Driessen
Interaction of Bacillus subtilis CsaA with SecA and precursor proteins.
Biochem J: 2000, 348 Pt 2(Pt 2);367-73
[PubMed:10816431]
[WorldCat.org]
(P p)
H Takamatsu, S Fuma, K Nakamura, Y Sadaie, A Shinkai, S Matsuyama, S Mizushima, K Yamane
In vivo and in vitro characterization of the secA gene product of Bacillus subtilis.
J Bacteriol: 1992, 174(13);4308-16
[PubMed:1385592]
[WorldCat.org]
[DOI]
(P p)
