RpoA

• Description: RNA polymerase alpha subunit
  
  

Gene name	rpoA
Synonyms
Essential	yes PubMed
Product	RNA polymerase alpha subunit
Function	transcription
Gene expression levels in SubtiExpress: rpoA
Interactions involving this protein in SubtInteract: RpoA
MW, pI	34 kDa, 4.593
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	rpsK, rplQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01430

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)

• Protein family: RNA polymerase alpha chain family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RpoA-RpoB-RpoC PubMed
  • CcpN-RpoA PubMed
  • RpoA (C-terminal domain) -Spx PubMed
  • MgsR-RpoA PubMed
  • CcpA-RpoA PubMed
  • RpoA-CodY PubMed

• Localization:

Database entries

• Structure: 1Z3E (C-terminal domain, complex with Spx)

• UniProt: P20429

• KEGG entry: [2]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon:

• Expression browser: rpoA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed