LytF

• Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
  
  

Gene name	lytF
Synonyms	cwlE, yhdD
Essential	no
Product	gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
Function	cell separation
Gene expression levels in SubtiExpress: lytF
Interactions involving this protein in SubtInteract: LytF
MW, pI	51 kDa, 10.41
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yhdC, nsrR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon, SlrR regulon

The gene

Basic information

• Locus tag: BSU09370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • IseA-LytF (catalytic domain) PubMed

• Localization:
  • secreted (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• Structure:

• UniProt: O07532

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytF PubMed

• Expression browser: lytF PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A791 ( lytF::spec), PubMed, available at BGSC
  • 1A792 ( lytF::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: