ArgF

• Description: ornithine carbamoyltransferase
  
  

Gene name	argF
Synonyms
Essential	no
Product	ornithine carbamoyltransferase
Function	biosynthesis of arginine
Gene expression levels in SubtiExpress: argF
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	34 kDa, 4.965
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	carB, yjzC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU11250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline (according to Swiss-Prot)

• Protein family: ATCase/OTCase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2EF0 (from Thermus thermophilus hb8, 48% identity, 63% similarity)

• UniProt: P18186

• KEGG entry: [3]

• E.C. number: 2.1.3.3

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF PubMed

• Expression browser: argF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC) PubMed

• Regulatory mechanism:
  • AhrC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A605 ( argF::erm), PubMed, available at BGSC
  • 1A606 ( argF::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)