AcuA

• Description: protein acetylase for the control of AcsA activity
  
  

Gene name	acuA
Synonyms
Essential	no
Product	Gcn5-related N-acetyltransferase
Function	control of AcsA activity
Gene expression levels in SubtiExpress: acuA
MW, pI	24 kDa, 5.028
Gene length, protein length	630 bp, 210 aa
Immediate neighbours	acsA, acuB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids, protein modification

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU29690

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acetylates (and thereby inactivates) AcsA on Lys-549 PubMed PubMed

• Protein family: acetyltransferase family (according to Swiss-Prot) Gcn5-relatedN-acetyltransferase PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39065

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: acuA-acuB-acuC PubMed

• Expression browser: acuA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (3.4-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • GP1211 (acuA::kan), available in Jörg Stülke's lab
  • GP1255 (acuA::kan yjcK::cat ynaD::spec yoaA::tet), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Jeffrey G Gardner, Jorge C Escalante-Semerena
In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase.
J Bacteriol: 2009, 191(6);1749-55
[PubMed:19136592] [WorldCat.org] [DOI] (I p)

Jeffrey G Gardner, Jorge C Escalante-Semerena
Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in Bacillus subtilis.
J Bacteriol: 2008, 190(14);5132-6
[PubMed:18487328] [WorldCat.org] [DOI] (I p)

Jeffrey G Gardner, Frank J Grundy, Tina M Henkin, Jorge C Escalante-Semerena
Control of acetyl-coenzyme A synthetase (AcsA) activity by acetylation/deacetylation without NAD(+) involvement in Bacillus subtilis.
J Bacteriol: 2006, 188(15);5460-8
[PubMed:16855235] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

F J Grundy, A J Turinsky, T M Henkin
Catabolite regulation of Bacillus subtilis acetate and acetoin utilization genes by CcpA.
J Bacteriol: 1994, 176(15);4527-33
[PubMed:7913927] [WorldCat.org] [DOI] (P p)

F J Grundy, D A Waters, T Y Takova, T M Henkin
Identification of genes involved in utilization of acetate and acetoin in Bacillus subtilis.
Mol Microbiol: 1993, 10(2);259-71
[PubMed:7934817] [WorldCat.org] [DOI] (P p)