Rnc

• Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
  
  

Gene name	rnc
Synonyms	rncS
Essential	yes
Product	endoribonuclease III
Function	cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	28 kDa, 8.076
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	acpA, smc
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU15930

Phenotypes of a mutant

essential PubMed

rnc is essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P51833

• KEGG entry: [3]

• E.C. number: 3.1.26.3

Expression and regulation

• Operon: rnc-smc-ftsY PubMed

• Expression browser: rnc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

Ian J MacRae, Jennifer A Doudna
Ribonuclease revisited: structural insights into ribonuclease III family enzymes.
Curr Opin Struct Biol: 2007, 17(1);138-45
[PubMed:17194582] [WorldCat.org] [DOI] (P p)

Xinhua Ji
Structural basis for non-catalytic and catalytic activities of ribonuclease III.
Acta Crystallogr D Biol Crystallogr: 2006, 62(Pt 8);933-40
[PubMed:16855311] [WorldCat.org] [DOI] (P p)

Djamel Drider, Ciarán Condon
The continuing story of endoribonuclease III.
J Mol Microbiol Biotechnol: 2004, 8(4);195-200
[PubMed:16179796] [WorldCat.org] [DOI] (P p)

R A Srivastava, N Srivastava
The multifaceted roles of the RNA processing enzyme ribonuclease III.
Indian J Biochem Biophys: 1996, 33(4);253-60
[PubMed:8936814] [WorldCat.org] (P p)