YkfB

• Description: L-Ala-D/L-Glu epimerase
  
  

Gene name	ykfB
Synonyms
Essential	no
Product	L-Ala-D/L-Glu epimerase
Function	cell wall metabolism
Gene expression levels in SubtiExpress: ykfB
MW, pI	39 kDa, 5.905
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	ykfA, ykfC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU12980

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1TKK (complex with substrate), 1JPM

• UniProt: O34508

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykfB PubMed

• Sigma factor:

• Regulation: repressed by CodY PubMed

• Regulatory mechanism: CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tiit Lukk, Ayano Sakai, Chakrapani Kalyanaraman, Shoshana D Brown, Heidi J Imker, Ling Song, Alexander A Fedorov, Elena V Fedorov, Rafael Toro, Brandan Hillerich, Ronald Seidel, Yury Patskovsky, Matthew W Vetting, Satish K Nair, Patricia C Babbitt, Steven C Almo, John A Gerlt, Matthew P Jacobson
Homology models guide discovery of diverse enzyme specificities among dipeptide epimerases in the enolase superfamily.
Proc Natl Acad Sci U S A: 2012, 109(11);4122-7
[PubMed:22392983] [WorldCat.org] [DOI] (I p)

Vadim A Klenchin, Dawn M Schmidt, John A Gerlt, Ivan Rayment
Evolution of enzymatic activities in the enolase superfamily: structure of a substrate-liganded complex of the L-Ala-D/L-Glu epimerase from Bacillus subtilis.
Biochemistry: 2004, 43(32);10370-8
[PubMed:15301535] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

A M Gulick, D M Schmidt, J A Gerlt, I Rayment
Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2001, 40(51);15716-24
[PubMed:11747448] [WorldCat.org] [DOI] (P p)

D M Schmidt, B K Hubbard, J A Gerlt
Evolution of enzymatic activities in the enolase superfamily: functional assignment of unknown proteins in Bacillus subtilis and Escherichia coli as L-Ala-D/L-Glu epimerases.
Biochemistry: 2001, 40(51);15707-15
[PubMed:11747447] [WorldCat.org] [DOI] (P p)