NatK

• Description: two-component sensor kinase, regulation of the natA-natB operon
  
  

Gene name	natK
Synonyms	yccG
Essential	no
Product	two-component sensor kinase
Function	regulation of the natA-natB operon
Interactions involving this protein in SubtInteract: NatK
MW, pI	34 kDa, 8.045
Gene length, protein length	954 bp, 318 aa
Immediate neighbours	yccF, natR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU02730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of NatR in response to sodium availability

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: three transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NatK-NatR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P70954

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: natK (according to DBTBS)

• Expression browser: natK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mitsuo Ogura, Kensuke Tsukahara, Kentaro Hayashi, Teruo Tanaka
The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion.
Microbiology (Reading): 2007, 153(Pt 3);667-675
[PubMed:17322186] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)