RsiX

• Description: anti-SigX
  
  

Gene name	rsiX
Synonyms	ypuN
Essential	no
Product	anti-SigX
Function	control of SigX activity
Interactions involving this protein in SubtInteract: RsiX
MW, pI	41 kDa, 5.452
Gene length, protein length	1104 bp, 368 aa
Immediate neighbours	aroC, sigX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigX regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU23090

Phenotypes of a mutant

• inactivation of rsiX strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RsiX-SigX PubMed

• Localization: cell membrane

Database entries

• Structure:

• UniProt: P35166

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigX-rsiX PubMed

• Expression browser: rsiX PubMed

• Sigma factor: SigA PubMed, SigX PubMed

• Regulation:

• Regulatory mechanism: activated by YvrHb PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Kei Asai, Yoshito Sadaie, Hirofumi Yoshikawa
Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors.
Microbiology (Reading): 2004, 150(Pt 3);591-599
[PubMed:14993308] [WorldCat.org] [DOI] (P p)

X Huang, J D Helmann
Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search.
J Mol Biol: 1998, 279(1);165-73
[PubMed:9636707] [WorldCat.org] [DOI] (P p)

S Brutsche, V Braun
SigX of Bacillus subtilis replaces the ECF sigma factor fecI of Escherichia coli and is inhibited by RsiX.
Mol Gen Genet: 1997, 256(4);416-25
[PubMed:9393439] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)