YwkE

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  • Description: similar to N5-glutamine methyltransferase that modifies peptide release factors

Gene name ywkE
Synonyms
Essential no
Product N5-glutamine methyltransferase
Function peptide release factor methylation
MW, pI 32 kDa, 4.89
Gene length, protein length 864 bp, 288 aa
Immediate neighbours ywkF, prfA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwkE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37000

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: hemK family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1NV8 (the protein from Thermotoga maritima) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Heidi L Schubert, John D Phillips, Christopher P Hill
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.
Biochemistry: 2003, 42(19);5592-9
[PubMed:12741815] [WorldCat.org] [DOI] (P p)

Valérie Heurgué-Hamard, Stéphanie Champ, Ake Engström, Måns Ehrenberg, Richard H Buckingham
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
EMBO J: 2002, 21(4);769-78
[PubMed:11847124] [WorldCat.org] [DOI] (P p)

Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Simon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada, Hachiro Inokuchi
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
Proc Natl Acad Sci U S A: 2002, 99(3);1473-8
[PubMed:11805295] [WorldCat.org] [DOI] (P p)