Gpr

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  • Description: spore protease (degradation of SASPs)

Gene name gpr
Synonyms
Essential no
Product spore protease (degradation of SASPs)
Function germination
MW, pI 40 kDa, 5.199
Gene length, protein length 1104 bp, 368 aa
Immediate neighbours spoIIP, rpsT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Gpr context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU25540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degradation of small, acid-soluble spore proteins (SASPs)
  • Protein family: peptidase A25 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation in the forespore (SigF, SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Thomas M Carroll, Peter Setlow
Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease.
J Bacteriol: 2005, 187(20);7119-25
[PubMed:16199582] [WorldCat.org] [DOI] (P p)

C Nessi, M J Jedrzejas, P Setlow
Structure and mechanism of action of the protease that degrades small, acid-soluble spore proteins during germination of spores of Bacillus species.
J Bacteriol: 1998, 180(19);5077-84
[PubMed:9748439] [WorldCat.org] [DOI] (P p)

L B Pedersen, C Nessi, P Setlow
Most of the propeptide is dispensable for stability and autoprocessing of the zymogen of the germination protease of spores of Bacillus species.
J Bacteriol: 1997, 179(5);1824-7
[PubMed:9045848] [WorldCat.org] [DOI] (P p)

B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid.
J Bacteriol: 1994, 176(22);7032-7
[PubMed:7961468] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro.
J Bacteriol: 1994, 176(17);5571-3
[PubMed:8071242] [WorldCat.org] [DOI] (P p)

Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species.
J Bacteriol: 1994, 176(10);2788-95
[PubMed:8188581] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, P Setlow
Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores.
J Bacteriol: 1993, 175(9);2568-77
[PubMed:8478323] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, M L Santiago-Lara, B Setlow, M D Sussman, P Setlow
Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination.
J Bacteriol: 1992, 174(3);807-14
[PubMed:1732215] [WorldCat.org] [DOI] (P p)

M D Sussman, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.
J Bacteriol: 1991, 173(1);291-300
[PubMed:1840582] [WorldCat.org] [DOI] (P p)