PbpA

• Description: penicillin-binding protein PBP 2A
  
  

Gene name	pbpA
Synonyms	yqgF
Essential	no
Product	penicillin-binding protein PBP 2A
Function	formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
Interactions involving this protein in SubtInteract: PbpA
MW, pI	79 kDa, 9.571
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	pstS, yqgE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis

The gene

Basic information

• Locus tag: BSU25000

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • folding requires PrsA PubMed

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P54488

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: pbpA PubMed

• Expression browser: pbpA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947