AlsT

• Description: amino acid carrier protein
  
  

Gene name	alsT
Synonyms
Essential	no
Product	amino acid carrier protein
Function	amino acid uptake
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	50 kDa, 9.39
Gene length, protein length	1395 bp, 465 aa
Immediate neighbours	ynfC, bglC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, putative amino acid transporter, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU18120

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sodium:alanine (SAF) symporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane PubMed

Database entries

• Structure:

• UniProt: Q45068

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: alsT PubMed

• Sigma factor:

• Regulation:
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)