CwlD

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  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlD
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function spore germination
MW, pI 26 kDa, 9.788
Gene length, protein length 711 bp, 237 aa
Immediate neighbours ybaK, salA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CwlD context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

germination

This gene is a member of the following regulons

LexA regulon, SigE regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU01530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
    • formation of muramic acid delta-lacton in peptidoglycan
  • Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Meghan E Gilmore, Dabanjan Bandyopadhyay, Amanda M Dean, Sarah D Linnstaedt, David L Popham
Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
J Bacteriol: 2004, 186(1);80-9
[PubMed:14679227] [WorldCat.org] [DOI] (P p)

Ann E Cowan, Dennis E Koppel, Barbara Setlow, Peter Setlow
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy.
Proc Natl Acad Sci U S A: 2003, 100(7);4209-14
[PubMed:12646705] [WorldCat.org] [DOI] (P p)

D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740] [WorldCat.org] [DOI] (P p)

J Sekiguchi, K Akeo, H Yamamoto, F K Khasanov, J C Alonso, A Kuroda
Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5582-9
[PubMed:7559346] [WorldCat.org] [DOI] (P p)