HtpX

• Description: similar to E.coli HtpX heat shock protease, stress-responsive membrane protease
  
  

Gene name	ykrL
Synonyms	htpX
Essential	no
Product	membrane protease
Function	quality control of membrane proteins
MW, pI	32 kDa, 10.157
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	ykrK, ktrD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

membrane proteins, proteolysis

This gene is a member of the following regulons

Rok regulon, YkrK regulon,

The gene

Basic information

• Locus tag: BSU13490

Phenotypes of a mutant

• increased sensitivity to membrane protein overproduction PubMed
• increased sensitivity to dissipation of the membrane potential by the addition of valinomycin PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M48B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31657

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by membrane protein overproduction (YkrK) PubMed
  • induced by dissipation of membrane potential (ΔΨ) by addition of a sublethal concentration of valinomycin PubMed
  • expression is increased due to mutations in resE, resB, sdhC, and sdhA PubMed
  • induced by salt stress PubMed

• Regulatory mechanism:
  • YkrK: transcription repression PubMed
  • Rok: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumila C Marciniak, Hein Trip, Fabrizia Fusetti, Oscar P Kuipers
Regulation of ykrL (htpX) by Rok and YkrK, a novel type of regulator in Bacillus subtilis.
J Bacteriol: 2012, 194(11);2837-45
[PubMed:22447908] [WorldCat.org] [DOI] (I p)