Des

• Description: phospholipid desaturase
  
  

Gene name	des
Synonyms	yocE
Essential	no
Product	phospholipid desaturase
Function	adaptation of membrane fluidity at low temperatures
Interactions involving this protein in SubtInteract: Des
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	40 kDa, 9.88
Gene length, protein length	1056 bp, 352 aa
Immediate neighbours	yocD, desK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

lipid metabolism/ other, cold stress proteins, membrane proteins

This gene is a member of the following regulons

DesR regulon

The gene

Basic information

• Locus tag: BSU19180

Phenotypes of a mutant

• • cold-sensitive PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type, this allows growth at low pressure PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: introduces double bonds into long chain fatty acids PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • Des receives electrons from ferredoxin (Fer) or from either of the two flavodoxins, YkuN or YkuP, the three proteins can functionally replace each other PubMed

• Effectors of protein activity:

• Interactions:
  • Des-Fer (electron transfer to Des) PubMed
  • Des-YkuN (electron transfer to Des) PubMed
  • Des-YkuP (electron transfer to Des) PubMed

• Localization: integral membrane protein PubMed

Database entries

• Structure:

• UniProt: O34653

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: des PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced upon cold shock (DesR) PubMed
  • repressed at increased membrane fluidity PubMed

• Regulatory mechanism:
  • two component system DesR-DesK: transcription activation PubMed, PubMed

• Additional information:
  • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage

References

Additional publications: PubMed