Ogt

• Description: O6-methylguanine DNA alkyltransferase
  
  

Gene name	ogt
Synonyms	dat
Essential	no
Product	O6-methylguanine DNA alkyltransferase
Function	DNA repair
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	18 kDa, 7.092
Gene length, protein length	495 bp, 165 aa
Immediate neighbours	kinE, mtnA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU13540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

• Protein family: MGMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1SFE (from E. coli, 36% identity, 51% similarity) PubMed

• UniProt: P11742

• KEGG entry: [3]

• E.C. number: 2.1.1.63

Expression and regulation

• Operon: kinE-ogt PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

J A Rafferty, J E Wibley, P Speers, I Hickson, G P Margison, P C Moody, K T Douglas
The potential role of glycine-160 of human O6-alkylguanine-DNA alkyltransferase in reaction with O6-benzylguanine as determined by site-directed mutagenesis and molecular modelling comparisons.
Biochim Biophys Acta: 1997, 1342(1);90-102
[PubMed:9366274] [WorldCat.org] [DOI] (P p)

F Morohoshi, N Munakata
Isolation of a Bacillus subtilis mutant defective in constitutive O6-alkylguanine-DNA alkyltransferase.
Mutat Res: 1990, 235(1);15-23
[PubMed:2105461] [WorldCat.org] [DOI] (P p)

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA alkyltransferase.
Nucleic Acids Res: 1989, 17(16);6531-43
[PubMed:2506524] [WorldCat.org] [DOI] (P p)