FabHA

• Description: beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells
  
  

Gene name	fabHA
Synonyms	yjaX , fabH1
Essential	no
Product	beta-ketoacyl-acyl carrier protein synthase III
Function	fatty acid biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	33 kDa, 5.045
Gene length, protein length	936 bp, 312 aa
Immediate neighbours	yjzB, fabF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

• Locus tag: BSU11330

Phenotypes of a mutant

• significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO₂ (according to Swiss-Prot)

• Protein family: fabH family (according to Swiss-Prot)

• Paralogous protein(s): FabHB, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1EBL (FabH from E. coli) PubMed

• UniProt: O34746

• KEGG entry: [3]

• E.C. number: 2.3.1.180, 2.3.1.41

Additional information

• affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis PubMed

Expression and regulation

• Operon: fabHA-fabF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • inhibited by cerulenin PubMed
  • induced upon fatty acid biosynthesis inhibition PubMed
  • expression is reduced when SigW is activated (by alkaline shock, polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100) PubMed

• Regulatory mechanism:
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed