YcnK

• Description: transcription repressor of the ycnJ gene
  
  

Gene name	ycnK
Synonyms
Essential	no
Product	transcription repressor
Function	regulation of copper uptake
MW, pI	21 kDa, 7.371
Gene length, protein length	570 bp, 190 aa
Immediate neighbours	ycnJ, ycnL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons

AbrB regulon

The YcnK regulon:

• ycnJ

The gene

Basic information

• Locus tag: BSU03960

Phenotypes of a mutant

elevated expression of ycnJ under conditions of copper excess PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: repression of ycnJ expression PubMed

• Protein family: DeoR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): copper acts as corepressor PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycnK (according to DBTBS)

• Sigma factor:

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Shashi Chillappagari, Marcus Miethke, Hein Trip, Oscar P Kuipers, Mohamed A Marahiel
Copper acquisition is mediated by YcnJ and regulated by YcnK and CsoR in Bacillus subtilis.
J Bacteriol: 2009, 191(7);2362-70
[PubMed:19168619] [WorldCat.org] [DOI] (I p)

Mélanie A Hamon, Nicola R Stanley, Robert A Britton, Alan D Grossman, Beth A Lazazzera
Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.
Mol Microbiol: 2004, 52(3);847-60
[PubMed:15101989] [WorldCat.org] [DOI] (P p)