RnpA

• Description: protein component of RNase P, metal-dependent 5' end maturation of precursor tRNAs
  
  

Gene name	rnpA
Synonyms
Essential	yes PubMed
Product	protein component of RNase P (substrate specificity)
Function	cleavage of precursor sequences from the 5' ends of pre-tRNAs
Interactions involving this protein in SubtInteract: RnpA
MW, pI	13 kDa, 10.804
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	spoIIIJ, rpmH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

Rnases, translation, essential genes

The gene

Basic information

• Locus tag: BSU41050

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to Swiss-Prot)

• Protein family: rnpA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • RNR motif: residues 60-68, stabilizes complexes with both the RNA part of RNase P (rnpB) and with pre-tRNA (rnpB-RnpA-pre-tRNA) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • RnpA-RpmH
  • RnpA-RnpB PubMed
  • RnpA-CshA PubMed

• Localization:

Database entries

• Structure:
  • 1AF6 PubMed
  • 3OKB (complex of RnpA with the RNA RnpB and tRNA, from Thermotoga maritima) PubMed

• UniProt: P25814

• KEGG entry: [2]

• E.C. number: 3.1.26.5

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Carol Fierke, University of Michigan, Ann Arbor, USA homepage
• Roland Hartmann, Marburg University, Germany homepage

References

Original Publications

Additional publications: PubMed