MhqO

• Description: hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone
  
  

Gene name	mhqO
Synonyms	ydfO
Essential	no
Product	hydroquinone-specific dioxygenase
Function	resistence to methyl-hydroxyquinone
MW, pI	34 kDa, 4.831
Gene length, protein length	936 bp, 312 aa
Immediate neighbours	mhqN, mhqP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

This gene is a member of the following regulons

MhqR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU05490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: extradiol ring-cleavage dioxygenase family (according to Swiss-Prot)

• Paralogous protein(s): MhqA, MhqE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3OAJ

• UniProt: P96693

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • mhqN-mhqO-mhqP PubMed
  • mhqO-mhqP PubMed

• Sigma factor:
  • mhqN: SigA PubMed
  • mhqO: SigB PubMed

• Regulation:
  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

• Regulatory mechanism:
  • MhqR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564] [WorldCat.org] [DOI] (P p)

Van Duy Nguyen, Carmen Wolf, Ulrike Mäder, Michael Lalk, Peter Langer, Ulrike Lindequist, Michael Hecker, Haike Antelmann
Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis.
Proteomics: 2007, 7(9);1391-408
[PubMed:17407181] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)