GlpT

• Description: glycerol-3-phosphate permease
  
  

Gene name	glpT
Synonyms	ybeE
Essential	no
Product	glycerol-3-phosphate permease
Function	glycerol-3-phosphate uptake
MW, pI	49 kDa, 9.366
Gene length, protein length	1332 bp, 444 aa
Immediate neighbours	glpQ, ybeF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, utilization of lipids, membrane proteins

This gene is a member of the following regulons

CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU02140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC37A family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37948

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glpT-glpQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (6.2-fold) (CcpA) PubMed
  • induced by glycerol (GlpP) PubMed
  • induced by phosphate limitation

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination via a protein-dependent RNA switch PubMed

• Additional information:

Biological materials

• Mutant: QB5437 (ermC), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

R P Nilsson, L Beijer, B Rutberg
The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);723-30
[PubMed:8012593] [WorldCat.org] [DOI] (P p)