McpB

• Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine
  
  

Gene name	mcpB
Synonyms
Essential	no
Product	methyl-accepting chemotaxis protein
Function	control of chemotaxis
Interactions involving this protein in SubtInteract: McpB
MW, pI	71 kDa, 5.13
Gene length, protein length	1986 bp, 662 aa
Immediate neighbours	tlpA, tgl
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU31260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): McpA

Extended information on the protein

• Kinetic information: K(D) for asparagine: 14 myM PubMed

• Domains:

• Modification: methylated at residues Gln371, Glu630, and Glu637 PubMed

• Cofactor(s):

• Effectors of protein activity: asparagine (binds to the part of McpB that is exposed to the exterior) PubMed

• Interactions: CheA-McpB PubMed, McpB-CheC PubMed

• Localization:
  • cell membrane PubMed
  • localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) PubMed

Database entries

• Structure:

• UniProt: P39215

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mcpB PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, McpB is present with 6,200 +/- 1,900 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: