GerBA

• Description: nutrient receptor, germination response to the combination of glucose, fructose, and KCl
  
  

Gene name	gerBA
Synonyms
Essential	no
Product	nutrient receptor
Function	germination response to the combination of glucose, fructose, and KCl
MW, pI	53 kDa, 7.295
Gene length, protein length	1446 bp, 482 aa
Immediate neighbours	pmi, gerBB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU35800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gerABKA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerBA-GerBB-GerBC (tentative receptor complex) PubMed
  • GerBA is part of the germinosome in the spore inner membrane PubMed

• Localization:
  • spore inner membrane PubMed

Database entries

• Structure:

• UniProt: P39569

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerBA-gerBB-gerBC PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Swaroopa Atluri, Katerina Ragkousi, Donna E Cortezzo, Peter Setlow
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor.
J Bacteriol: 2006, 188(1);28-36
[PubMed:16352818] [WorldCat.org] [DOI] (P p)

Takao Igarashi, Peter Setlow
Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores.
J Bacteriol: 2005, 187(7);2513-8
[PubMed:15774895] [WorldCat.org] [DOI] (P p)

Rosa-Martha Cabrera-Martinez, Federico Tovar-Rojo, Venkata Ramana Vepachedu, Peter Setlow
Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis.
J Bacteriol: 2003, 185(8);2457-64
[PubMed:12670969] [WorldCat.org] [DOI] (P p)

M Paidhungat, P Setlow
Localization of a germinant receptor protein (GerBA) to the inner membrane of Bacillus subtilis spores.
J Bacteriol: 2001, 183(13);3982-90
[PubMed:11395462] [WorldCat.org] [DOI] (P p)

M Paidhungat, P Setlow
Isolation and characterization of mutations in Bacillus subtilis that allow spore germination in the novel germinant D-alanine.
J Bacteriol: 1999, 181(11);3341-50
[PubMed:10348844] [WorldCat.org] [DOI] (P p)

B M Corfe, A Moir, D Popham, P Setlow
Analysis of the expression and regulation of the gerB spore germination operon of Bacillus subtilis 168.
Microbiology (Reading): 1994, 140 ( Pt 11);3079-83
[PubMed:7812448] [WorldCat.org] [DOI] (P p)

B M Corfe, R L Sammons, D A Smith, C Mauël
The gerB region of the Bacillus subtilis 168 chromosome encodes a homologue of the gerA spore germination operon.
Microbiology (Reading): 1994, 140 ( Pt 3);471-8
[PubMed:8012571] [WorldCat.org] [DOI] (P p)