NagB

• Description: glucosamine-6-phosphate deaminase
  
  

Gene name	nagB
Synonyms
Essential	no
Product	glucosamine-6-phosphate deaminase
Function	N-acetylglucosamine utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling
MW, pI	26 kDa, 5.717
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	nagA, nagR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

NagR regulon

The gene

Basic information

• Locus tag: BSU35020

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃ (according to Swiss-Prot)

• Protein family: NagB subfamily (according to Swiss-Prot)

• Paralogous protein(s): GamA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BKXPubMed

• UniProt: O35000

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Florence Vincent, Gideon J Davies, James A Brannigan
Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?
J Biol Chem: 2005, 280(20);19649-55
[PubMed:15755726] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)