LicB

• Description: trigger enzyme: lichenan-specific phosphotransferase system, EIIB component of the PTS
  
  

Gene name	licB
Synonyms	celA
Essential	no
Product	trigger enzyme: lichenan-specific phosphotransferase system, EIIB component
Function	lichenan uptake and phosphorylation, control of LicR activity
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	10 kDa, 6.314
Gene length, protein length	306 bp, 102 aa
Immediate neighbours	licC, licR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LicR regulon

The gene

Basic information

• Locus tag: BSU38590

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, lactose permease (Lac) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-37 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LicA-LicB, LicB-LicC, LicB-LicR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P46318

• KEGG entry: [2]

• E.C. number: 2.7.1.69 9

Expression and regulation

• Operon: licB-licC-licA-licH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by oligomeric ß-glucosides (LicR)PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: